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1994-08-09
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38 lines
Document 0181
DOCN M9480181
TI Interleukin-2-dependent T-cell lines established from paroxysmal
nocturnal hemoglobinuria patients.
DT 9410
AU Nakakuma H; Nagakura S; Horikawa K; Hidaka M; Kawaguchi T; Iwamoto N;
Sanada I; Kagimoto T; Takatsuki K; Second Department of Internal
Medicine, Kumamoto University; School of Medicine, Japan.
SO Blood. 1994 Jul 1;84(1):309-14. Unique Identifier : AIDSLINE
MED/94289717
AB Peripheral blood T lymphocytes obtained from two patients with
paroxysmal nocturnal hemoglobinuria (PNH) were immortalized with human
T-lymphotropic virus type 1 (HTLV-1). These cells showed interleukin-2
(IL-2)-dependent cell growth in culture. Cell surface analysis showed
that they had the phenotype of a helper/inducer T subset that was
positive for CD2, CD3, and CD4, but negative for CD8, similar to adult
T-cell leukemia cells induced by HTLV-1. These cell lines lacked
glycosylphosphatidylinositol (GPI)-anchored proteins, CDw52, CD55
(decay-accelerating factor; DAF), and CD59 on the cell surface, whereas
intracellular DAF protein was detected. These T-subset cell lines with a
PNH phenotype did not synthesize GPI anchor, whereas a control cell
line, similarly prepared from the T cells of a healthy volunteer,
produced the anchor. The control cells expressed CDw52, DAF, and CD59 on
the cell surface and showed the phenotype of a helper/inducer subset.
Southern blot analysis confirmed the clonality of each cell line. These
CD4+ T-cell lines with a PNH phenotype and a subset-matched control
counterpart could be a useful model for PNH investigation.
DE Antigens, CD/ANALYSIS Cell Line
Glycosylphosphatidylinositols/BIOSYNTHESIS Hemoglobinuria,
Paroxysmal/*IMMUNOLOGY Human HTLV-I/GENETICS
Interleukin-2/*PHARMACOLOGY Karyotyping Lymphocyte Transformation/DRUG
EFFECTS Membrane Glycoproteins/ANALYSIS Support, Non-U.S. Gov't
T-Lymphocytes/*PHYSIOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).